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human pulmonary microvascular endothelial cells (hpmec, cat. 3000)  (ScienCell)

 
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    ScienCell human pulmonary microvascular endothelial cells (hpmec, cat. 3000)
    A Assessment of cytotoxicity of the expressed viroporins in human pulmonary alveolar epithelial cells (HPAEpiCs) 24 h after lentiviral infection by the LDH release (left column) and DRAQ7 incorporation (right column). B Effects of the viroporins on HMGB-1 release by HPAEpiCs. C Assessment of cytotoxicity of the expressed viroporins in human pulmonary <t>microvascular</t> <t>endothelial</t> cells (HPMECs) 24 h after lentiviral infection by the LDH release (left column) and DRAQ7 incorporation (right column). D Effects of the viroporins on HMGB-1 release by HPMECs. E Immunoblots for the inflammasome proteins (upper panel) and their respective densitometric analysis (lower panel) analyzed in HPMECs. C- control, mCH-mCherry. Graphs represent means ± SEM, n = 3. Two groups comparisons were analyzed with t-test. Multiple comparisons were performed with two-way ANOVA test. * p < 0.05, ** p < 0.01.
    Human Pulmonary Microvascular Endothelial Cells (Hpmec, Cat. 3000), supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hpmecs+3000/human+pulmonary+microvascular+endothelial+cells++hpmecs+/pmc11045860-212-0-20
    Average 90 stars, based on 1 article reviews
    human pulmonary microvascular endothelial cells (hpmec, cat. 3000) - by Bioz Stars, 2026-10
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    1) Product Images from "SARS-CoV-2 and its ORF3a, E and M viroporins activate inflammasome in human macrophages and induce of IL-1 α in pulmonary epithelial and endothelial cells"

    Article Title: SARS-CoV-2 and its ORF3a, E and M viroporins activate inflammasome in human macrophages and induce of IL-1 α in pulmonary epithelial and endothelial cells

    Journal: Cell Death Discovery

    doi: 10.1038/s41420-024-01966-9

    A Assessment of cytotoxicity of the expressed viroporins in human pulmonary alveolar epithelial cells (HPAEpiCs) 24 h after lentiviral infection by the LDH release (left column) and DRAQ7 incorporation (right column). B Effects of the viroporins on HMGB-1 release by HPAEpiCs. C Assessment of cytotoxicity of the expressed viroporins in human pulmonary microvascular endothelial cells (HPMECs) 24 h after lentiviral infection by the LDH release (left column) and DRAQ7 incorporation (right column). D Effects of the viroporins on HMGB-1 release by HPMECs. E Immunoblots for the inflammasome proteins (upper panel) and their respective densitometric analysis (lower panel) analyzed in HPMECs. C- control, mCH-mCherry. Graphs represent means ± SEM, n = 3. Two groups comparisons were analyzed with t-test. Multiple comparisons were performed with two-way ANOVA test. * p < 0.05, ** p < 0.01.
    Figure Legend Snippet: A Assessment of cytotoxicity of the expressed viroporins in human pulmonary alveolar epithelial cells (HPAEpiCs) 24 h after lentiviral infection by the LDH release (left column) and DRAQ7 incorporation (right column). B Effects of the viroporins on HMGB-1 release by HPAEpiCs. C Assessment of cytotoxicity of the expressed viroporins in human pulmonary microvascular endothelial cells (HPMECs) 24 h after lentiviral infection by the LDH release (left column) and DRAQ7 incorporation (right column). D Effects of the viroporins on HMGB-1 release by HPMECs. E Immunoblots for the inflammasome proteins (upper panel) and their respective densitometric analysis (lower panel) analyzed in HPMECs. C- control, mCH-mCherry. Graphs represent means ± SEM, n = 3. Two groups comparisons were analyzed with t-test. Multiple comparisons were performed with two-way ANOVA test. * p < 0.05, ** p < 0.01.

    Techniques Used: Infection, Western Blot

    Related Articles

    Immunofluorescence:

    Article Title: Effect of lipopolysaccharide on angiotensin II type 1 receptor expression and function in human pulmonary microvascular endothelial cells.
    Article Snippet: HPMECs (cat. no. 3000), endothelial cell medium (ECM) and endothelial cell growth supplement (ECGS) were obtained from ScienCell Research Laboratories (Carlsbad, CA, USA).



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    A Assessment of cytotoxicity of the expressed viroporins in human pulmonary alveolar epithelial cells (HPAEpiCs) 24 h after lentiviral infection by the LDH release (left column) and DRAQ7 incorporation (right column). B Effects of the viroporins on HMGB-1 release by HPAEpiCs. C Assessment of cytotoxicity of the expressed viroporins in human pulmonary <t>microvascular</t> <t>endothelial</t> cells (HPMECs) 24 h after lentiviral infection by the LDH release (left column) and DRAQ7 incorporation (right column). D Effects of the viroporins on HMGB-1 release by HPMECs. E Immunoblots for the inflammasome proteins (upper panel) and their respective densitometric analysis (lower panel) analyzed in HPMECs. C- control, mCH-mCherry. Graphs represent means ± SEM, n = 3. Two groups comparisons were analyzed with t-test. Multiple comparisons were performed with two-way ANOVA test. * p < 0.05, ** p < 0.01.
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    A Assessment of cytotoxicity of the expressed viroporins in human pulmonary alveolar epithelial cells (HPAEpiCs) 24 h after lentiviral infection by the LDH release (left column) and DRAQ7 incorporation (right column). B Effects of the viroporins on HMGB-1 release by HPAEpiCs. C Assessment of cytotoxicity of the expressed viroporins in human pulmonary <t>microvascular</t> <t>endothelial</t> cells (HPMECs) 24 h after lentiviral infection by the LDH release (left column) and DRAQ7 incorporation (right column). D Effects of the viroporins on HMGB-1 release by HPMECs. E Immunoblots for the inflammasome proteins (upper panel) and their respective densitometric analysis (lower panel) analyzed in HPMECs. C- control, mCH-mCherry. Graphs represent means ± SEM, n = 3. Two groups comparisons were analyzed with t-test. Multiple comparisons were performed with two-way ANOVA test. * p < 0.05, ** p < 0.01.
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    A Assessment of cytotoxicity of the expressed viroporins in human pulmonary alveolar epithelial cells (HPAEpiCs) 24 h after lentiviral infection by the LDH release (left column) and DRAQ7 incorporation (right column). B Effects of the viroporins on HMGB-1 release by HPAEpiCs. C Assessment of cytotoxicity of the expressed viroporins in human pulmonary <t>microvascular</t> <t>endothelial</t> cells (HPMECs) 24 h after lentiviral infection by the LDH release (left column) and DRAQ7 incorporation (right column). D Effects of the viroporins on HMGB-1 release by HPMECs. E Immunoblots for the inflammasome proteins (upper panel) and their respective densitometric analysis (lower panel) analyzed in HPMECs. C- control, mCH-mCherry. Graphs represent means ± SEM, n = 3. Two groups comparisons were analyzed with t-test. Multiple comparisons were performed with two-way ANOVA test. * p < 0.05, ** p < 0.01.
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    A Assessment of cytotoxicity of the expressed viroporins in human pulmonary alveolar epithelial cells (HPAEpiCs) 24 h after lentiviral infection by the LDH release (left column) and DRAQ7 incorporation (right column). B Effects of the viroporins on HMGB-1 release by HPAEpiCs. C Assessment of cytotoxicity of the expressed viroporins in human pulmonary <t>microvascular</t> <t>endothelial</t> cells (HPMECs) 24 h after lentiviral infection by the LDH release (left column) and DRAQ7 incorporation (right column). D Effects of the viroporins on HMGB-1 release by HPMECs. E Immunoblots for the inflammasome proteins (upper panel) and their respective densitometric analysis (lower panel) analyzed in HPMECs. C- control, mCH-mCherry. Graphs represent means ± SEM, n = 3. Two groups comparisons were analyzed with t-test. Multiple comparisons were performed with two-way ANOVA test. * p < 0.05, ** p < 0.01.
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    A Assessment of cytotoxicity of the expressed viroporins in human pulmonary alveolar epithelial cells (HPAEpiCs) 24 h after lentiviral infection by the LDH release (left column) and DRAQ7 incorporation (right column). B Effects of the viroporins on HMGB-1 release by HPAEpiCs. C Assessment of cytotoxicity of the expressed viroporins in human pulmonary microvascular endothelial cells (HPMECs) 24 h after lentiviral infection by the LDH release (left column) and DRAQ7 incorporation (right column). D Effects of the viroporins on HMGB-1 release by HPMECs. E Immunoblots for the inflammasome proteins (upper panel) and their respective densitometric analysis (lower panel) analyzed in HPMECs. C- control, mCH-mCherry. Graphs represent means ± SEM, n = 3. Two groups comparisons were analyzed with t-test. Multiple comparisons were performed with two-way ANOVA test. * p < 0.05, ** p < 0.01.

    Journal: Cell Death Discovery

    Article Title: SARS-CoV-2 and its ORF3a, E and M viroporins activate inflammasome in human macrophages and induce of IL-1 α in pulmonary epithelial and endothelial cells

    doi: 10.1038/s41420-024-01966-9

    Figure Lengend Snippet: A Assessment of cytotoxicity of the expressed viroporins in human pulmonary alveolar epithelial cells (HPAEpiCs) 24 h after lentiviral infection by the LDH release (left column) and DRAQ7 incorporation (right column). B Effects of the viroporins on HMGB-1 release by HPAEpiCs. C Assessment of cytotoxicity of the expressed viroporins in human pulmonary microvascular endothelial cells (HPMECs) 24 h after lentiviral infection by the LDH release (left column) and DRAQ7 incorporation (right column). D Effects of the viroporins on HMGB-1 release by HPMECs. E Immunoblots for the inflammasome proteins (upper panel) and their respective densitometric analysis (lower panel) analyzed in HPMECs. C- control, mCH-mCherry. Graphs represent means ± SEM, n = 3. Two groups comparisons were analyzed with t-test. Multiple comparisons were performed with two-way ANOVA test. * p < 0.05, ** p < 0.01.

    Article Snippet: Human Pulmonary Microvascular Endothelial Cells (HPMEC, Cat. 3000) and Human Pulmonary Alveolar Epithelial Cells (HPAEpiC, Cat. 3200) were obtained from ScienCell Research Laboratories (CA, USA).

    Techniques: Infection, Western Blot